In vitro expression of recombinant in-frame deletions. (A) Quantification of total VWF:Ag as a percentage of WT. (B) Quantification of total VWF:Ag as a percentage of WT (expressed as a proportion of lysate and supernatant). (C) VWF secretion ratios normalized to WT (ratio, 1.0). (D) Effective secretion of VWF normalized to WT (100%). Data were analyzed using 1-way ANOVA and Dunnett’s posttest multiple comparison with WT (**P < .01; ***P < .001; ****P < .0001). Mean values for n = 3 triplicate measurements are shown (bars indicate standard error of the mean). Het, heterozygous; Hom, homozygous; ns, not significant.