Widefield fluorescence microscopy of recombinant homozygous in-frame deletions. HEK293 cells were transfected with WT rVWF only (A-D) and either rVWF3del (E-H), rVWF32-34del (I-L), or rVWF33-34del (M-P). Cells were fixed and stained for VWF (green), ER (red), and Golgi (magenta). Scale bars, 5 μm.