The 5-hmC gain is enriched in hypoxia-induced genes. Enrichment of all 5-hmC (A), 5-hmC gain (B), and 5-hmC loss (C) in upregulated genes at day 3, day 7, and day 10 of in vitro erythropoiesis. (D) Gene ontology term enrichment of day 7 upregulated genes containing regions with 5-hmC gain (green bar in panel B). All terms shown have a false discovery rate <0.05. (E) Gene ontology term enrichment of day 10 upregulated genes containing regions with 5-hmC gain (dark green bar in panel B). All terms shown have a false discovery rate <0.05. N = 2 for RNA-sequencing experiments. VEGF, vascular endothelial growth factor.