Endothelial cells secrete IL-6 and GM-CSF in response to P aeruginosa. A multiplexed enzyme-linked immunosorbent assay screen was run on lumen-conditioned media from 6 conditions with different combinations of neutrophils, P aeruginosa, and an endothelium. (A) The log2 fold change of protein levels over the endothelial lumen-only condition are displayed as a heat map. Blue indicates lower expression and red indicates increased expression. Condition combinations are indicated on the top of the heat map. Factors measured are listed on the left of the heat map. Four factors (VEGFD, VEGFA, P-selectin, and RANKL) were below the level of detection and are not displayed. (B) The levels of GM-CSF expressed as a fold change over the condition with only an endothelial lumen were plotted. Bars represent least-squared mean plus SEM. Condition combinations are indicated below the graph. (C) The levels of IL-6 expressed as a fold change over the condition with only an endothelial lumen were plotted. Bars represent least-squared mean plus SEM. Condition combinations are indicated below the graph. Data in B and C are the same as for these proteins in A, but displayed with statistics. All data represent 4 independent replicates, with media from 6 lumens pooled per replicate. Asterisks show significance relative to all other conditions, with the level of significance represented as the lowest level for any condition. *P < .05 and **P < .01.