CTCF differential occupancy. (A-E) MA plots of differentially bound CTCF sites between AML^all and NBM (A), AML^TET2mut and NBM (B), AML^TET2wt and NBM (C), AML^TET2mut and AML^TET2wt (D), and AML^NPM1mut and AML^NPM1wt (E) (FDR <0.05). (F) Venn diagram showing DBC overlap for comparisons between AML^all vs NBM (pink circle), AML^TET2mut vs AML^TET2wt (blue circle), and AML^NPM1mut and AML^NPM1wt (green circle). (G) Distribution of gained and lost CTCF binding over genomic features for AML^all and AML^TET2mut specific DBCs. (H) Enrichment of differentially bound CTCF peaks in promoters (left) and enhancers (right) compared with all detected CTCF peaks. (I-J) Scatterplot of GO terms of gained DBCs in AML^all vs NBM (I) and AML^TET2mut vs AML^TET2wt (J). Circle color indicates P value (−log₁₀P value) and circle size the frequency of the GO terms. (K) Motif analysis of all DBCs in AML^all vs NBM. (L) ChIP quantitative PCR using anti-CTCF in K562-CTCF knockdown cells (*P < .05; Student t test). (M) ChIP quantitative PCR using anti-RUNX1 in K562-CTCF knockdown cells; scramble control was set to 1 (dashed line), and values were normalized against scramble control (*P < .05; Student t test).