Analysis of the hb genes in the nprl3-linked L fluviatilis hb locus. (A) Schematic drawing (to scale) of the L fluviatilis hb locus. Exons of hb genes are shown as red boxes. To indicate the direction of transcription, gene names are positioned next to the first exon. (B) Expression of the L fluviatilis hb genes assessed by RT-PCR. Red asterisks indicate fragments of expected size for cDNA amplicons; blue asterisks indicate fragments of expected size for genomic amplicons. (C) Proteomic analysis of L fluviatilis HB proteins in larvae and adults. Peptides identified by mass spectrometry are indicated by colored bars: light blue, peptides unique to HB1-HB4; lavender, peptides unique to HB1 and HB4; magenta, peptides unique to HB2 and HB3; red, peptides unique to HB5; orange, peptides unique to HB6. tr, transposon; u1 and u2, predicted genes.