EGF-mediated activation of DNA-PKcs is dependent on Akt. (A) Representative microscopic images of p-Akt in nonirradiated (Non-IRR) and irradiated (IRR) KSL cells cultured in media, with or without EGF, for 5 minutes (scale bar, 10 µm) (left panel). p-Akt MFI in KSL cells for each condition (n = 34-37 cells per group, Student t test) (right panel). (B) Representative microscopic images of p-Akt in Non-IRR and irradiated KSL cells cultured with media with or without EGF and with or without MK2206 for 5 minutes (scale bar, 10 µm) (left panel). p-Akt MFI in KSL cells for each condition (n = 26-44 cells per group, 1-way ANOVA) (right panel). (C) Representative microscopic images of γ-H2AX foci in the conditions shown at 1 hour of culture (scale bar, 10 µm) (left panel). Numbers of foci per KSL cell for each condition (n = 20 fields of view per group, 1-way ANOVA) (right panel). (D) Representative microscopic images of p-DNA-PKcs foci in the conditions shown (scale bar, 10 µm). Numbers of p-DNA-PKcs foci per KSL cell for each condition (n = 20 fields of view per group, 1-way ANOVA) (right panel). (E) CFCs from KSL cells irradiated with 300 cGy and cultured in media with or without EGF and with or without MK2206 (n = 6 per group). Data are mean ± SEM. *P < .05, **P < .01, ***P < .001, ****P < .0001, 2-way ANOVA. DAPI, 4′,6-diamidino-2-phenylindole. E+M, EGF + MK2206.