Ability of common HLA-DRB1 subtypes to present fungal antigens to antigen- and allelically mismatched panfungal T cells. (A) Flow cytometry analysis of TNF-α expression in panfungal T cells cultured for 6 hours with nonpulsed or fungal lysate–pulsed MoDCs when the DCs presenting fungi antigens share 2 HLA-DRB1 antigens, only 1 HLA-DRB1 antigen, and only 1 HLA-DRB1 antigen but with different allele subtype and neither of the HLA-DRB1 antigens (mismatched) with the panfungal T cells. Results are means of 2 to 4 experiments (±SEM) and shown as percentage of CD4⁺ T cells. (A) *P ≤ .05 partially matched vs mismatched and allele subtype missmatched vs mismatched; ΦP ≤ .001 matched vs partially matched, partially matched with allele subtype missmatched and missmatched (unpaired, 2-tailed Student t test). (B-F) Flow cytometry analysis of TNF-α expression in panfungal CD4⁺ T cells with common HLA types (DRB1 01:01, 04:01, 11:01, 13:01, or 15:01) cultured for 6 hours with allelic-mismatched nonpulsed or fungal lysate–pulsed DCs; DCs express (B) HLA-DRB1 01:02; (C) HLA-DRB1 04:03, 04:04, 04:05; (D) HLA-DRB1 11:04; (E) HLA-DRB1 13:02; and (F) HLA-DRB1 15:02. Results are means of 2 to 6 experiments (±SEM) and shown as percentage of CD4⁺ T cells (**P ≤ .01, *P ≤ .05 DC control vs DC pulsed; paired, 2-tailed Student t test).