Cytokines and gene expression analysis. (A) Concentrations of cytokines released within the OvCa-Chip relative to the Control-Chip (MCP-1, IL-8, TNF-α, and IL-6), which showed a significant increase in time (n = 3 individual experiments). (B) Relative expression of different endothelial genes in OvCa-Chips vs Control-Chips. The percentage expression of respective genes (relative to GAPDH) were normalized with respect to the percentage expression measured in Control-Chips. Error bars indicate the mean ± SEM; n = 3 individual experiments. Two-tailed paired Student t test; *P < .05.