Functional screening of CD8^pos TAA-specific T-cell clones derived from HLA-A*02:01^pos donors. (A) Minimal numbers of unique T-cell clones per TAA specificity isolated from 18 donors. (B) Summary of classification of unique TAA-specific T-cell clones based on IFN-γ production after overnight stimulation with TAP-deficient T2 cells exogenously loaded with a titration of the respective peptide (R:S ratio, 1:6). (C) Results of this functional screening per TAA specificity. Clones depicted in green were classified as intermediate avidity, clones depicted in blue as low avidity, clones depicted in gray as not functional, and clones depicted in purple as T2-reactive T-cell clones. Representative examples of unique clones are depicted. NS, not stimulated.