Figure 5.
Relative expression of TAA in different target cell lines and primary samples measured by RT-qPCR. The expression of the indicated TAA is calculated relative to expression of 2 household genes (ACTB and GAPDH). A relative expression of 1 means equal expression compared with the average expression of these household genes. (A) The expression of WT1 could not be determined in EBV-LCL transduced with the retroviral vector encoding the codon-optimized protein sequence of WT1, as a suitable primer was not available. Expression of WT1 in EBV-LCL transduced mock, THP-1, ALL BLM, and K562 cell line and 2 representative primary AML and ALL samples are depicted. (B) Expression of PRAME in EBV-LCL transduced mock and PRAME, THP-1, U266, K562 cell line, and 2 representative primary AML samples are depicted. (C) Expression of NY-eso-1 in EBV-LCL transduced mock and NY-eso-1, MM cell lines RPMI 8226, OPM-2, and U266, and 1 representative primary MM sample are depicted.

Relative expression of TAA in different target cell lines and primary samples measured by RT-qPCR. The expression of the indicated TAA is calculated relative to expression of 2 household genes (ACTB and GAPDH). A relative expression of 1 means equal expression compared with the average expression of these household genes. (A) The expression of WT1 could not be determined in EBV-LCL transduced with the retroviral vector encoding the codon-optimized protein sequence of WT1, as a suitable primer was not available. Expression of WT1 in EBV-LCL transduced mock, THP-1, ALL BLM, and K562 cell line and 2 representative primary AML and ALL samples are depicted. (B) Expression of PRAME in EBV-LCL transduced mock and PRAME, THP-1, U266, K562 cell line, and 2 representative primary AML samples are depicted. (C) Expression of NY-eso-1 in EBV-LCL transduced mock and NY-eso-1, MM cell lines RPMI 8226, OPM-2, and U266, and 1 representative primary MM sample are depicted.

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