Functional reactivity of the NY-eso-1-SLL–specific T-cell clone of intermediate avidity against cell lines endogenously overexpressing NY-eso-1. NY-eso-1-SLL clone “Donor D-TRBV9” was tested against EBV-LCL transduced with the empty vector (mock) or with the vector encoding the full protein sequence of NY-eso-1, MM cell lines RPMI 8226 transduced with the empty vector (mock) or with HLA-A*02:01, MM cell line OPM-2 transduced with the empty vector (mock) or with HLA-A*02:01, MM cell line U266, and a primary MM sample. An allo-HLA-A*02:01–reactive T-cell clone was included as positive control for HLA-A*02:01 expression and costimulatory capacity of the targets. T2 or K562 transduced with HLA-A*02:01 and loaded with peptide were included as positive control. The HLA-A*02:01 and TAA expression of the targets is indicated as negative (–) or positive (+) below the x-axis. (A) IFN-γ production was measured by using an ELISA after overnight stimulation (R:S ratio, 1:6). Representative example of 3 experiments is shown. (B) Cytotoxic capacity was measured in a standard ⁵¹chromium-release assay at different E:T ratios. Mean of triplicates with standard deviation is depicted.