Conditional gene inactivation of Tek in mice. (A-B) Tek^lox/lox and Tek^iECKO mice were injected intraperitoneally with tamoxifen on 5 consecutive days and analyzed 3 days later. (A) Total lysates of lung, heart and skin were immunoblotted for Tie-2, VE-cadherin and α-tubulin. (B) Cremaster whole mounts were stained for Tie-2 and PECAM-1. Scale bars, 50 µm.