Loss of hepatic Trf exacerbates liver damage induced by an HID. (A) Non-heme iron was measured in the indicated tissues of control and Trf-LKO mice (n = 4 mice/group) after 8 weeks on an HID. (B) Perls Prussian blue staining of liver sections obtained from HID-fed control and Trf-LKO mice; the scale bars represent 100 μm. (C) Serum ALT levels were measured in control and Trf-LKO mice fed either an SID or an HID (n = 4 mice/group). (D) 4-HNE, Sirius Red, and Masson’s trichrome staining were performed on liver sections obtained from HID-fed control and Trf-LKO mice; the scale bars represent 100 μm. Hepatic MDA, thiol, and SOD activity (E) and hepatic Slc7a11 and Gpx4 mRNA (F) were measured in SID-fed and HID-fed control mice and Trf-LKO mice (n = 4 mice/group). *P < .05, **P < .01, Student t test. n.s., not significant.