Fer-1 treatment rescues iron overload‒induced ferroptosis in Trf-LKO mice. Hepatic non-heme iron (A), serum ALT (B), hepatic MDA (C), and hepatic thiol (D) levels were measured in HID-fed control mice and Trf-LKO mice treated with saline or Fer-1 (n = 3-6 mice/group). (E) Liver sections obtained from HID-fed control and Trf-LKO mice treated with saline or Fer-1 were stained with 4-HNE, Sirius Red, and Masson’s trichrome; the scale bars represent 100 μm. (F) Quantitative analyses for Sirius red and Masson’s trichrome staining in the indicated groups. (G) Hepatic mRNA levels of the fibrotic genes Col1a1 and PDGF were measured in the indicated groups. *P < .05, **P < .01, one-way analysis of variance with Tukey’s post hoc test. n.s., not significant.