Loss of hepatic Slc39a14 protects against ferroptosis in Trf-LKO mice. (A) Hepatic non-heme iron was measured in control (Trf^fl/fl), Trf-LKO, and Trf/Slc39a14 double-knockout (DKO) mice fed an SID (n = 4 mice/group). (B) Representative images of Perls Prussian blue‒stained liver sections obtained from SID-fed control, Trf-LKO, and DKO mice; the scale bars represent 100 μm. Hepatic non-heme iron (C), thiol (D), and MDA (E) levels were measured in HID-fed control, Trf-LKO, and DKO mice (n = 4 mice/group). (F) Representative images of liver sections obtained from HID-fed control, Trf-LKO, and DKO mice and stained with Perls Prussian blue, 4-HNE, Sirius Red, and Masson’s trichrome; the scale bars represent 100 μm. *P < .05 and **P < .01, one-way analysis of variance with Tukey’s post hoc test.