Effects of IMiDs on TSLP-induced responses in mDCs. Blood mDCs were treated with media alone, TSLP, TSLP + LEN, TSLP + POM, or TSLP + BOR. (A,C) After 24 hours of culture, CCL17 concentration in the supernatants was analyzed by an ELISA. (B) After 4 hours of mDC culture (with TSLP in the presence of LEN or POM at 0.3 μM), STAT6 and IRF4 mRNA in mDCs were analyzed by real-time PCR. Each experiment was performed using DCs from a single donor, and data are shown as the mean ± SEM of 6 independent donors. Statistical significance was determined using a paired Student t test (*P < .05, **P < .01). Comparisons between data obtained for vehicle controls (indicated as TSLP alone) and for each concentration of the indicated drugs.