Effect of IMiDs on the function of DCs to total, naïve, and memory Th2 memory cells. Total (A), naïve (B), and CRTH2⁺CD4⁺ (C) T cells were cultured for 7 days with allogeneic mDCs pretreated with media alone, LPS, TSLP, TSLP + 0.3 μM LEN, or TSLP + 0.3 μM POM for 24 hours. After culture, cytokine production by primed CD4⁺ T cells was measured in supernatants after restimulation for 24 hours with anti-CD3 and soluble anti-CD28 antibodies at a concentration of 10⁶ cells/mL. A single experiment was performed using DCs from 1 donor and T cells from another allogenic donor, and data are the means ± SEM of 4 (A-B) and 5 (C) independent experiments. Statistical significance was determined using a paired Student t test (*P < .05, **P < .01).