Figure 4.
Upregulation of phosphorylated BCL-2 in in vitro expanded Treg-A and Treg-B. (A) The percentage of early (Viability Dye eFluor 780− Annexin V+) and late (Viability Dye eFluor 780+ Annexin V+) apoptotic cells in FasL-induced Treg-A and Treg-B before and after treatment with 20 IU/mL of human IL-2. Error bars represent mean ± SD. **P ≤ .01. (B) GSEA enrichment plot shows the significant enrichment of gene set for negative regulation of extrinsic apoptotic signaling pathway in AA-expanded Treg-A as compared with Treg-A before expansion. (C) Western blots show the BCL-2 and p-BCL-2 (Ser70) protein expression levels in HD and AA-expanded Treg-A and Treg-B in comparison with HD total Treg preexpansion. β-ACTIN protein level is used as a loading control. (D) Densitometric quantification of p-BCL-2 (Ser70) protein expression level normalized to β-ACTIN. Error bars represent mean ± SD. *P ≤ .05.

Upregulation of phosphorylated BCL-2 in in vitro expanded Treg-A and Treg-B. (A) The percentage of early (Viability Dye eFluor 780− Annexin V+) and late (Viability Dye eFluor 780+ Annexin V+) apoptotic cells in FasL-induced Treg-A and Treg-B before and after treatment with 20 IU/mL of human IL-2. Error bars represent mean ± SD. **P ≤ .01. (B) GSEA enrichment plot shows the significant enrichment of gene set for negative regulation of extrinsic apoptotic signaling pathway in AA-expanded Treg-A as compared with Treg-A before expansion. (C) Western blots show the BCL-2 and p-BCL-2 (Ser70) protein expression levels in HD and AA-expanded Treg-A and Treg-B in comparison with HD total Treg preexpansion. β-ACTIN protein level is used as a loading control. (D) Densitometric quantification of p-BCL-2 (Ser70) protein expression level normalized to β-ACTIN. Error bars represent mean ± SD. *P ≤ .05.

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