Effects of ^superFVa and rhFVIIa on thrombin generation in NHP spiked with FXa inhibitors. Thrombin generation was measured in NHP in the presence of the FXa inhibitors, apixaban or rivaroxaban. (A,C) Representative graphs showing the change in thrombin generation curves as the result of increasing concentrations of ^superFVa (0-400 nM) in the presence or absence of rhFVIIa (40 nM) in NHP spiked with apixaban (200 nM) (A) or rivaroxaban (200 nM) (C). (B,D) Enhancement of ETP by increasing concentrations of ^superFVa (0-400 nM) in the absence (Δ) or presence (○) of rhFVIIa (40 nM) in NHP spiked with apixaban (200 nM) (B) or rivaroxaban (200 nM) (D). Additional controls shown are NHP with (□) and without (◇) apixaban or rivaroxaban. (B,D) Peak height and lag time of are shown in supplemental Figure 4. Error bars represent standard error of the mean (n ≥ 3).