Reversal of DOAC-induced bleeding with ^superFVa and rhFVIIa in wild-type BalbC mice. Mice were treated with apixaban (8 mg/kg) (A), rivaroxaban (40 mg/kg) (B), and dabigatran (0.4 mg/kg) (C) by IV tail vein injection and bleeding was measured for 20 minutes after tail clip. Blood loss was expressed in microliters of blood per gram (g) mouse. Increasing doses of ^superFVa, rhFVIIa, or rhFVIIa in combination with ^superFVa were injected retro-orbitally 30 minutes after DOAC administration and 5 minutes before tail clip. Error bars represent SEM (n = 8-12 per group). P values were determined by Kruskal-Wallis followed by a 2-tailed Mann-Whitney U test and values ≤.05 were considered statistically significant.