Anti-HLA Fc Asparagine (N)-297-linked glycan structures are similar in TRALI donors compared with control donors. (A) The conserved IgG-Fc glycan core structure consists of a bi-antennary glycan core of GlcNAc and mannose. In addition, glycans can be variably present such as fucose, galactose, sialic acid, and bisecting GlcNAc. Relative expression levels of major IgG-Fc Asn297 glycoforms for both total anti-HLA IgG1 (x-axis) and antigen-specific HLA IgG1 (y-axis) for 11 TRALI donor samples (B,D,F,H) and 20 control donor samples (C,E,G,I). HLA specificities include HLA-A02 HLA-A24, HLA-B07, and HLA-B35. Samples were analyzed for Fc fucosylation (B-C), galactosylation (D-E), sialylation (F-G), and bisection (H-I). The statistical outcome between 2-tailed paired Student t test analysis of total IgG1 vs specific antibodies is listed in each panel. The diagonal, dotted line represents the equal ratio between total IgG1 and the specific antibody. **P < .01, ***P < .001.