Neutrophils from HH patients are not iron loaded. (A) Plasma iron, transferrin (Tf) saturation, and ferritin in healthy donors (n = 16) and HH patients (n = 11). (B) Western blot of FPN, FTH, and β-actin on total protein extracts from neutrophils isolated from healthy donors and HH patients. (C) Neutrophil intracellular iron content quantification by atomic absorption (ETAAS) normalized by sodium content in healthy donors (n = 3) versus HH patients (n = 3). (D) Western blot band intensity quantification for FPN and FTH normalized by the healthy donor mean band intensity and expressed in arbitrary units. All results are shown as mean ± SEM. ns, not significant.