Neutrophils from HH patients are primed. (A) Plasma TNF-α, VCAM-1, and ICAM-1 in the plasma of HH patients (n = 23; red squares) vs healthy donors (n = 18; blue circles). (B) Correlations between plasma TNF-α and transferrin saturation (Transf Sat) or between plasma TNF-α and area under the curve (AUC) of the oxidative burst upon stimulation with fMLF, OZ, or PMA for each patient. (C) Analysis by flow cytometry of CD62L surface expression on neutrophils isolated from HH patients (n = 4) vs healthy donors (n = 3) as measured by mean fluorescence intensity (MFI) and expressed in fold change. (D-E) Evaluation of the phagocytosis capacity (number of phagocytized S aureus bioparticles) in neutrophils from HH patients (red) vs healthy donors (blue) (n ≥ 6; data from 3 independent experiments). (D) Analysis of the percentage of phagocytic neutrophils over time. (E) Comparison of the MFI between neutrophils from HH patients (red) vs healthy donors (blue). The normality of each data batch was assessed to determine the appropriate statistic test to use. Mann-Whitney U test was used for analysis of TNF-α and ICAM-1 in panel A, unpaired Student t test for panel E and VCAM-1 graph in panel A, and 2-way ANOVA followed by a Sidak test in panel D. All results are shown as mean ± SEM. *P < .05; **P < .01.