DCs derived from NK-cell–primed monocytes through cell-to-cell contact via NKp30 drive the polarization of IFN-γ and IL-17A-producers type 17 CD8⁺T cells. DCs derived from NK-cell–primed monocytes (NK-iDCs) were cocultured with purified naive allogeneic T cells for 5 days. DCs derived from monocytes alone (iDCs) or primed by NK cells through a Transwell membrane (NK//iDCs) were used as controls. By the fifth day of coculture, T-cell phenotype and capacity to produce cytokines after PMA plus ionomycin stimuli was assessed by flow cytometry. (A) Frequency of T-bet⁺IFN-γ⁺ and RORγT⁺IL-17A⁺ within CD25⁺CD8⁺ T cells stimulated by DCs at different conditions. (B) Production (iMFI: frequency multiplied by MFI) of IFN-γ and IL-17A by CD25⁺CD8⁺ T cells stimulated by DCs at different conditions. (C) Gating strategy for the analysis of polarized CD25⁺CD8⁺ T cells. (D) Comparison of different types of CD25⁺CD8⁺ T cells polarized by DCs from different conditions. (E) Frequency of IFN-γ and IL-17A producers type 17 CD8⁺ T cells (T-bet⁺IFN-γ⁺RORγT⁺IL-17A⁺) stimulated by control DCs and DCs derived from monocytes primed by NK cells, in the presence of anti-NKp30 (α-NKp30) or IgG1,κ isotype control (IgG1,κ). (F) tSNE analysis of concatenated samples (all conditions combined) and split by iDCs and NK-iDCs overlaid samples, respectively; MFI statistic heatmaps of the concatenated sample for DC markers. Error bars represent standard deviation. Data are representative of at least 2 or more independent experiments combined; n = 2-3 HDs per experiment. Statistical comparisons were performed with the unpaired Student t test with Welchs correction. *P < .05, **P < .01, ***P < .001.