Patients with classical NK-cell deficiency (GATA2 mutations) have low frequencies of Clec4D⁺SLAMF7⁺DCs and reduced Tc17/ IFN-γ responses. PBMCs derived from blood of 7 patients bearing mutations on the GATA2 gene were analyzed by flow cytometry. PBMCs from 2 patients (indicated by black and green dots) were analyzed at a single-cell level; only for 1 patient (green dot), samples before and after bone marrow transplant were obtained; PBMCs from HD were used as controls. (A) Frequencies of CD3⁻CD56⁺ (NK cells) and Lin⁻CD14⁺ cells (monocytes) in PBMCs from patients with GATA2 deficiency. (B) tSNE analysis of the different populations found in PBMCs before and after transplant. (C) Heatmap of genes differentially expressed on the population of myeloid cells, before and after transplant. (D) Frequency of HLA-DR⁺CD14^lowIL-15RA⁺ DCs from GATA2 patients and HD controls. (E) Heatmap of genes differentially expressed on the population of CD8 T cells, before and after transplant. (F) Dot plots of CD45RO⁺CD8⁺ T cells from a GATA2 patient before and after transplant with the corresponding HD control. (G) Frequency of CD45RO⁺CD8⁺ T cells from GATA2 patients and HD controls. PBMCs from GATA2 patients and HDs were stimulated for 6 hours by PMA and ionomycin. (H) Frequency of Tc1, Tc17/IFN-γ and Tc17 cells from GATA2 patients and HD controls. (I) Gene expression of CXCR3 and KLRB1 on CCL5 expressing CD8 T cells from GATA2 patients and HD controls. Error bars represent standard deviation. Data are representative of at least 2 or more independent experiments combined; n = 2-3 HDs per experiment. Statistical comparisons were performed with the unpaired Student t test with Welch correction.