Immunodetection of endogenously expressed Hjv in the liver of Tmprss6^−/− mice that express exogenous Mt2. (A) Representative images of western blot analysis for endogenously expressed Hjv, β-actin, and introduced Mt2, Mt2^S762A, Mt2^mask, or FLAG-tagged Hjv (fHjv) in the whole liver extracts (250 µg protein) of wild-type (WT), Tmprss6^−/−, and Hjv^−/− mice. (B) Representative images of western blot analysis for endogenously expressed Hjv, β-actin, and introduced Mt2 or Mt2^I286F in the whole liver extracts (250 µg protein) of WT and Tmprss6^−/− mice. (C) Representative images of western blot analysis for endogenously expressed Hjv, and introduced Mt2, Mt2^S762A, Mt2^mask, or Hjv in the liver membrane preparation (250 µg protein) of WT, Tmprss6^−/−, and WT 129S6 mice expressing exogenous FLAG-tagged Hjv (fHjv). IB, immunoblotting; n.s., nonspecific band.