Figure 4.
![Louvain clustering of CyTOF data reveals that CART-transfected NK cells maintain NK cell repertoire diversity. NK cells (500 000 cells per well) were transfected with GFP-encoding mRNA via L2000 (31 ng of mRNA per well), electroporation (31 ng per well [Elec] or 10 000 ng of mRNA per well [Elec high]), or CART O5:N6:A9 (31 ng of mRNA per well). (A) UMAP dimensionality reduction plot colored by identified clusters. (B) Heatmap of mean marker expression per cluster, with rows clustered hierarchically. (C) Proportions of cells belonging to each cluster.](https://ash.silverchair-cdn.com/ash/content_public/journal/bloodadvances/4/17/10.1182_bloodadvances.2020002355/1/advancesadv2020002355f4.png?Expires=1736044659&Signature=Mqm3TIOq2kuRswSGFd3GF0yhQJXR-0cZy05oth1uIoizz0REfvzN0wvTlLqnL04elgrYqj-IGBkdAQX1IXND0M28e-d0LsbIlyv4ezU2iifQVv4z20OpprLTnlQFyWmKIdssHCM0FYTbGuMdKbXH10A~pwiTXsTXnCMLXvGQiw9wIeX29t9Qstjxl0swld1qzFHVxeG44bmG2QtJ4X1Em8dN3vMpla2YJjGhsgr5GcWocpoysp~JtSTKXHihg0~wM14j~ZL4la~gNTvbsLlV01Pwe79LcAbN-8ElhiE7OYNSu5aGsPC91rFsoxcaUAHwbMKBlQD5sad2Ypm0R92uVw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Louvain clustering of CyTOF data reveals that CART-transfected NK cells maintain NK cell repertoire diversity. NK cells (500 000 cells per well) were transfected with GFP-encoding mRNA via L2000 (31 ng of mRNA per well), electroporation (31 ng per well [Elec] or 10 000 ng of mRNA per well [Elec high]), or CART O5:N6:A9 (31 ng of mRNA per well). (A) UMAP dimensionality reduction plot colored by identified clusters. (B) Heatmap of mean marker expression per cluster, with rows clustered hierarchically. (C) Proportions of cells belonging to each cluster.