iNKT cell reconstitution in the gut after transplantation. (A) The frequency of the iNKT TRAV11-TRAJ18 gene usage in the donor graft and GI tract of recipients. The percent sequence reads that iNKT account for in the total sequence for each mouse is shown. The middle horizontal bar (in black) indicates the mean values in each group at days 9 to 11 or days 18 to 32 (n > 6 mice per group pooled from 2 experiments; 2-tailed Student t test). (B) Representative dot plots of iNKT staining by flow cytometry for gut and spleen. CD3⁺TCRab⁺ T cells (upper panels) and TCRab⁺CD1d-tetramer⁺ iNKT cells (lower panels) identified by flow cytometry in single-cell suspension of the spleen and gut of allogeneic BALB/c recipient mice (vs syngeneic C56BL/6; gray bar) days 9 to 11. Quantification of the percent iNKT in the spleen (C) and GI tract (D). Both T and iNKT cells in BALB/c recipients are significantly reduced as a percentage of lymphocytes and in absolute count of mononuclear cells (n > 5 mice per group pooled from 2 experiments; 2-tailed Student t test.).