Figure 5.
12-LOX–derived DPAn-6oxylipins, 11-HpDPAn-6and 14-HpDPAn-6, inhibit human platelet accumulation on a collagen-coated surface under arterial shear flow conditions. Whole blood collected in a sodium-citrate vacutainer tube was incubated with dimethyl sulfoxide (DMSO) or 100 nM to 5 μM DPAn-6 (n = 6) or 250 nM to 2.5 μM DPAn-6 (n = 5) (A), 11-HpDPAn-6 (n = 5) (B), or 14-HpDPAn-6 (n = 5) (C) for 5 minutes at 37°C prior to perfusion over a collagen-coated surface at arterial shear rate (1800/s) for 5 minutes (original magnification, ×20). Representative figures for each condition are shown to the left of each composite graph. Data are mean ± SEM. Statistical significance calculated using 2-way ANOVA. MFI, mean fluorescence intensity.

12-LOX–derived DPAn-6oxylipins, 11-HpDPAn-6and 14-HpDPAn-6, inhibit human platelet accumulation on a collagen-coated surface under arterial shear flow conditions. Whole blood collected in a sodium-citrate vacutainer tube was incubated with dimethyl sulfoxide (DMSO) or 100 nM to 5 μM DPAn-6 (n = 6) or 250 nM to 2.5 μM DPAn-6 (n = 5) (A), 11-HpDPAn-6 (n = 5) (B), or 14-HpDPAn-6 (n = 5) (C) for 5 minutes at 37°C prior to perfusion over a collagen-coated surface at arterial shear rate (1800/s) for 5 minutes (original magnification, ×20). Representative figures for each condition are shown to the left of each composite graph. Data are mean ± SEM. Statistical significance calculated using 2-way ANOVA. MFI, mean fluorescence intensity.

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