PPARα mediates the inhibitory effects of DPA_n-6metabolites on platelet aggregation. Washed platelets from WT and PPARα^−/− mice were incubated with DPA_n-6 (A), 11-HpDPA_n-6 (B), or 14-HpDPA_n-6 (C), ranging from 0.5 to 5 μM, for 5 minutes prior to EC₈₀ collagen-induced aggregation. Data are means ± SEM. *P < .05, ***P < .001, ****P < .0001, 1-way ANOVA.