Protein expression of members in LSC-specific selected pathways. Heat map representation of log2 fold-change protein expression of indicated comparisons showing members of the KEGG cell adhesion molecules (A), Hallmark oxidative phosphorylation (C), and KEGG spliceosome (D) pathways. (B) Validation of selected cell adhesion surface proteins by flow cytometry in n = 15 AML samples (n = 7 FLT3-ITD/NPM1-mut and n = 8 FLT3-WT/NPM1-WT cases) and n = 5 HSPC samples. (E) Number of significantly spliced exons (false discovery rate [FDR] <0.1, Δ-PSI >0.1) in LSC vs blast comparison of FLT3-ITD/NPM1-mut (HD20, DD06, DD07, DD08) and FLT3-WT/NPM1-WT (HD48, DD10, DD13, with or without outlier sample B06) groups. (F) Δ-PSI values of common and significantly sliced exons in both groups (left), quantified in violin plot representation (right). Paired Student t test. PSI, percent-spliced-in as determined by Whippet.¹⁵