Analysis of gene expression by RNA sequencing. (A) Heatmap showing gene regulatory network enrichment scores as analyzed by CellNet²⁹  for FANCA-expressing (Dox) and FANCA-deficient (No dox) hematopoietic cells from day 8 EHT culture. Individual profiles for each of 3 biologic replicates from both cell types are shown. (B) GSEA was used to query the Molecular Signatures Database,³⁰  and relevant terms enriched in FANCA-expressing cells relative to FANCA-deficient cells are presented. In all GSEA analyses, a preranked transcript list was used that was compiled from 3 biologic replicates of sequencing for both FANCA-expressing and FANCA-deficient hematopoietic cells. (C) GSEA was used to query gene sets enriched in human umbilical cord blood HSCs relative to non-HSCs.³³  (D) GSEA was used to query the Molecular Signatures Database, and terms enriched in FANCA-deficient cells relative to FANCA-expressing cells are presented. (E) Expression of erythroid genes in FANCA-expressing vs FANCA-deficient hematopoietic cells (analysis with a Student t test, n = 3 biologic replicates, results presented as mean ± standard deviation; P values are shown).