Erythroid differentiation of FANCA-expressing or -deficient IPSC-derived hematopoietic cells. (A) Day 8 nonadherent cells from EHT culture were cultured in a cytokine cocktail containing erythropoietin for the indicated periods of time after the initiation of EHT culture, when erythroid differentiation was measured by flow cytometry for CD71 and GLYA expression. (B) Within the GLYA⁺ gate, cell surface expression of CD49d and Band 3 was measured by flow cytometry. Stages of erythroid differentiation were quantified by gating as described previously.⁴²  (C) Stages of erythroid differentiation were quantified at day 14, and each stage was compared by a Student t test (n = 5 biologic replicates, results presented as mean ± SEM). (D) Representative morphology of day 14 erythroid differentiation culture (scale bar, 10 μm).