Figure 3.
BH3-mimetic drugs targeting BCL-XL induce cell death in ENKTL and CAEBV cell lines. (A) Expression levels of cellular BCL-2, BCL-XL, MCL-1, BIM, PUMA, NOXA, BAK, and BAX were detected by western blot analysis. Probing for HSP70 was used as a protein loading control. The Burkitt lymphoma–derived cell lines, BL41 and Raji, and LCL were used as controls. Representative blots are shown. (B) Cells were treated for 48 hours with 0.5 µM or 5 µM of the indicated BH3-mimetic drugs ABT-199 (BCL-2 specific), ABT-737 (inhibits BCL-2, BCL-XL, and BCL-W), A-1331852 (BCL-XL specific), or S63845 (MCL-1 specific). Cells were then stained with annexin V and PI and their viability quantitated by flow cytometry. Data shown are normalized to DMSO-treated control cells and presented as mean ± SD of 3 independent experiments.

BH3-mimetic drugs targeting BCL-XL induce cell death in ENKTL and CAEBV cell lines. (A) Expression levels of cellular BCL-2, BCL-XL, MCL-1, BIM, PUMA, NOXA, BAK, and BAX were detected by western blot analysis. Probing for HSP70 was used as a protein loading control. The Burkitt lymphoma–derived cell lines, BL41 and Raji, and LCL were used as controls. Representative blots are shown. (B) Cells were treated for 48 hours with 0.5 µM or 5 µM of the indicated BH3-mimetic drugs ABT-199 (BCL-2 specific), ABT-737 (inhibits BCL-2, BCL-XL, and BCL-W), A-1331852 (BCL-XL specific), or S63845 (MCL-1 specific). Cells were then stained with annexin V and PI and their viability quantitated by flow cytometry. Data shown are normalized to DMSO-treated control cells and presented as mean ± SD of 3 independent experiments.

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