miR-125a-5p regulates platelet count in vivo. LNAs to miR-125a-5p (125a LNA) or negative LNA control (negC LNA) were administered intraperitoneally to C57BL/6 WT mice every other day for 9 days. On day 10, mice were euthanized and assays performed. Fold changes in murine platelet (A) and BM (B) miR-125a-5p levels compared with negC LNA control (n = 8 in each group). (C) Platelet counts from both groups of mice (n = 8). (D) Quantification of von Willebrand factor (VWF)-positive MKs in BM of mice femurs per area (scored blinded as to the treatment group) (n = 3 for negC LNA and n = 4 for 125a LNA). (E) Representative image of VWF immunohistochemical staining of BM (n = 4 in each group). (F) Representative hematoxylin-and-eosin staining of BM (n = 4 in each group). Arrows indicate MK. (E-F) Scale bar, 50 μm.