American Society of Hematology

Figure 2.

In vitro platelet activation dose/response curves. Flow cytometric analysis of (A,C,E) P-selectin expression and (B,D,F) integrin αIIbβ3 activation of platelets from matched WT, RGS18−/−, RGS10−/−, and RGS10−/−18−/− mice. Platelets were stimulated with increasing doses of PAR4P (AYPGKF) (A-B), ADP (C-D), and TxA2 analog (U46619) (E-F) and gated by forward scatter/side scatter and CD41 positivity. At least 4 measurements were collected per genotype per condition (mean ± SEM). See supplemental Figure 2 for statistical comparisons between genotypes. FITC, fluorescein isothiocyanate; MFI, mean fluorescence intensity; PE, phycoerythrin.

In vitro platelet activation dose/response curves. Flow cytometric analysis of (A,C,E) P-selectin expression and (B,D,F) integrin α_IIbβ₃ activation of platelets from matched WT, RGS18^−/−, RGS10^−/−, and RGS10^−/−18^−/− mice. Platelets were stimulated with increasing doses of PAR4P (AYPGKF) (A-B), ADP (C-D), and TxA₂ analog (U46619) (E-F) and gated by forward scatter/side scatter and CD41 positivity. At least 4 measurements were collected per genotype per condition (mean ± SEM). See supplemental Figure 2 for statistical comparisons between genotypes. FITC, fluorescein isothiocyanate; MFI, mean fluorescence intensity; PE, phycoerythrin.

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