The effect of STAT6 inhibition on transcription in MF and SS tumor samples. (A) Volcano plots comparing gene expression in AS1517499-treated for 48 hours vs untreated MF skin tumors (n = 3) and blood SS cell samples (n = 4). FC, fold change. Blue dots and lines identify the genes scored as differentially expressed (Log₂FC > ±0.58 and P < .05). (B) Venn diagram showing the relationship between numbers of DEGs in MF or SS samples after treatment with STAT6 inhibitor or medium alone. The total number of DEGs in SS or MF is given in parenthesis. The number of DEGs unique to SS or MF or shared between them are shown within the diagram; the numbers of genes that were upregulated (up) or downregulated (down) in MF/SS after STAT6 inhibition are also indicated. (C) Heat maps showing a selection of the top DEGs after STAT6 inhibition in individual SS or MF tumor samples. (D) Pathway analysis by Ingenuity of the most significant DEGs commonly up- or downregulated in MF and SS samples after STAT6 inhibition.