STAT6 regulates gene expression of TAMs in the TME of advanced stage MF. (A) Transcriptomes of macrophages by scRNAseq from individual MF skin tumors (n = 3, 2312 cells) and NS samples (n = 4, 322 cells), color-coded by subject, (B) revealing 9 discrete Louvain clusters. (C) Gene expression from the 9 discrete Louvain clusters in panel B, showing examples of highly significant DEGs in each of the clusters. Cluster numbers are indicated at the top. Each column represents a cell. (D) Dot-plot showing the proportion of cells and the scaled average gene differential expression of the macrophage tumor-associated genes selected in panel C. (E) Bar plot showing the differential expression of the genes selected in panel C after STAT6 inhibition for 48 hours of MF skin tumors (Log₂FC > ± 0.58 and P < .05). (F) Three-color immunofluorescence microscopy showing coexpression of CD163, MRC1, and CCL17 or CTSC, in advanced-stage MF skin explants (n = 5) cultured for 5 days with/without AS1517499. Representative examples are shown at ×600, inset double the scale.