Responding patients exhibit changes in T-cell memory subsets, NK cells, CD16⁺NK cells, and γδ T cells with long-term treatment. Flow cytometric analysis of CD4⁺ and CD8⁺ memory subsets (A), NK cells, CD16⁺ NK cells, γδ T cells, and CD14⁺ cells at baseline (n = 13) (B), week 4 (W4; n = 13), week 16 (W16; n = 14), 12 months (M12; n = 13), 18 months (M18; n = 13), 24 months (M24; n = 11), 36 months (M36; n = 10), and 48 months (M48; n = 7). P values from 2-way analysis of variance with Dunnett’s multiple comparisons to B (A) and Kruskal-Wallis test with Dunn’s multiple comparisons to B (B). *P < .05, **P < .01, ***P < .001.