Recurrent gene mutations in MDS/MPN. (A) Distribution of adjusted VAFs across recurrently mutated genes individually (left) or grouped according to their functional category (right). The median-adjusted VAF of signaling genes was significantly lower than that of epigenetic regulators (P < .0001), splicing factors (P < .0001), transcription factors (0.0007), and other genes (P < .0001). The median-adjusted VAF of transcription factors was significantly lower than those of the epigenetic regulators (P < .0001) and splicing factors (P < .0001). (B) Bar plot showing the frequency of recurrently mutated genes among the different MDS/MPN subtypes.