Molecular landscape and clonal architecture of CMML. (A) Oncoplot showing recurrently mutated genes and ancestry in CMML: blue depicts ancestral mutations (darker blue represents cases with 2 ancestral mutations in the same gene, mainly corresponding to biallelic TET2 mutations); yellow depicts secondary mutations and the intensity of the shade indicates the size of the VAF, where darker yellow represents mutations with higher VAFs that have probably been acquired earlier; orange depicts cases with 2 mutations in the same gene in which 1 mutation is ancestral and the other is secondary (mainly corresponding to biallelic TET2 mutations). (B) Frequency of ancestral/secondary mutations per gene in all patients with CMML (n = 1191; left) and in patients who present with at least 2 different clones (n = 77; right). (C) The clonal architecture of CMML. Black arrows depict most common events; gray arrows depict events that are recurrent but occur less frequently.