Molecular landscape and clonal architecture of aCML. (A) Oncoplot showing recurrently mutated genes and ancestry in aCML: pink depicts ancestral mutations (darker pink represents cases with 2 ancestral mutations in the same gene); yellow depicts secondary mutations, and the intensity of the shade indicates the size of the VAF (darker yellow represents mutations with higher VAFs that have probably been acquired earlier); purple depicts cases with 2 mutations in the same gene in which 1 mutation is ancestral and the other is secondary. (B) Frequency of ancestral/secondary mutations per gene in all patients with aCML (n = 71; left) and in patients who present with at least 2 different clones (n = 49; right). (C) The clonal architecture of aCML. Black arrows depict most common events; gray arrows depict events that are recurrent but occur less frequently.