Molecular landscape and clonal architecture of MDS/MPN-RS-T. (A) Oncoplot showing recurrently mutated genes and ancestry in MDS/MPN-RS-T: green depicts ancestral mutations (darker green represents cases with 2 ancestral mutations in the same gene); yellow depicts secondary mutations, and the intensity of the shade indicates the size of the VAF, where darker yellow represents mutations with higher VAF which have probably been acquired earlier; orange depicts cases with 2 mutations in the same gene, where 1 mutation is ancestral and the other is secondary. (B) Frequency of ancestral/secondary mutations per gene in all patients with MDS/MPN-RS-T (n = 71; left) and in patients who present with at least 2 different clones (n = 4; right). (C) The clonal architecture of MDS/MPN-RS-T. Black arrows depict most common events; gray arrows depict events that are recurrent but occur less frequently.