Molecular landscape and clonal architecture of MDS/MPN-U. (A) Oncoplot showing recurrently mutated genes and ancestry in MDS/MPN-U: gray depicts ancestral mutations (darker gray represents cases with 2 ancestral mutations in the same gene); yellow depicts secondary mutations, and the intensity of the shade indicates the size of the VAF, where darker yellow represents mutations with higher VAFs that probably have been acquired earlier; orange depicts cases with 2 mutations in the same gene, where 1 mutation is ancestral and the other is secondary. Patients are divided according to their molecular subtype (top of the barplot). (B) Frequency of ancestral/secondary mutations per gene in all patients with MDS/MPN-U (n = 106; left) and in those who present with at least 2 different clones (n = 65; right).