Scribble scaffolds Yap1 and Cdc42 in the cytoplasm of HSC. (A) Venn diagram highlighting the number of differentially regulated genes in common when comparing Mx1Cre;Wt and Mx1Cre;Scribble^Δ/Δ and comparing Mx1Cre;Wt and Mx1Cre;Taz^Δ/Δ;Yap1^Δ/Δ HSC. (B) Gene ontology (GO) pathway analysis of the common differentially regulated genes between 2 independent analysis of Mx1Cre;Wt to Mx1Cre;Scribble^Δ/Δ HSC and of Mx1Cre;Wt to Mx1Cre;Taz^Δ/Δ;Yap1^Δ/Δ HSC. Numbers represent the number of genes within each GO pathway that are differentially regulated. (C) Heat map depicting the differential regulation of common genes between Mx1Cre; Scribble^Δ/Δ and Mx1Cre;Taz^Δ/Δ;Yap1^Δ/Δ HSC that pertain to the Rho guanyl nucleotide exchange factor activity and small GTPase activity gene ontology pathways. Proximity ligation assay (PLA) detection of endogenous (D) Yap1/Cdc42 and (E) Yap1/Cdc42-GTP interactions in HSC. The detected dimers are pseudo-colored in red. Nuclei are counterstained with DAPI and merged images are shown in the right micrographs. Scale bar is 5 µm. (F) Frequency of HSC in which PLA signal depicted in panels D and E was found in relation or not with polarization. *P < .05 and **P < .01 between Vav1Cre;Wt and Vav1Cre;Scribble^Δ/Δ; ^##P < .01 between Cdc42 and Cdc42-GTP frequencies. (G) Cartoon representing the overall polarization status between Scribble, Yap1, and Cdc42 in Wt cells emphasizing the loss of copolarization and asymmetry in Scribble^Δ/Δ HSC.