![As Red Cells Become Oxygenated in the Lungs, a Nitric Oxide (NO) Group is Covalently Bound to the Highly Conserved Cys93 Residue in the ß-Chain of Hemoglobin. In this high oxygen affinity state (the relaxed, R-state), SNO-Hgb is unreactive, but upon Hgb deoxygenation in the periphery and the consequent transition to the low oxygen affinity state (the tense, T-state), SNO-Hgb can react with red cell thiols (e.g., glutathione [GSH] or anion exchanger-1 [AE-1]) via transnitrosation and thereby transmit a vasodilatory signal (RSNO) out of the red cell.](https://ash.silverchair-cdn.com/ash/content_public/journal/thehematologist/6/1/10.1182_hem.v6.1.6175/2/6175a.jpeg?Expires=1735547983&Signature=uo7C7H3FtfWyEvTVBBaJOxwLn6ZgJEVPeIGYEF-P7mRGlzOr6Ssv2Qq5DS4z0rdxVt63-LWAnAAyal7dKMQ4l-yblORVPgbEfBjoTD0ahR0DTSG9FCEhVmKswrnZPggoOMWsA9sQiUWdQZ0J9Kr9G~OxQUj-ww4pYSYjUcT1OwzIesK96I~L4WMdyKWLq8iJml0oNoinzai06jXINyyEtILnQIVh544cyEobLzkslrQxCLyju8y7bo117N6yHET93AyFG7qtvxHWvo6MIp0gxkEnewkCHV9tY2PCsfaNkuqy5oOS8QQnTYOffd-d0Bkdo5WmycOK8WgnzCIVH70WdQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
As Red Cells Become Oxygenated in the Lungs, a Nitric Oxide (NO) Group is Covalently Bound to the Highly Conserved Cys93 Residue in the ß-Chain of Hemoglobin. In this high oxygen affinity state (the relaxed, R-state), SNO-Hgb is unreactive, but upon Hgb deoxygenation in the periphery and the consequent transition to the low oxygen affinity state (the tense, T-state), SNO-Hgb can react with red cell thiols (e.g., glutathione [GSH] or anion exchanger-1 [AE-1]) via transnitrosation and thereby transmit a vasodilatory signal (RSNO) out of the red cell.
