Impact of kinase inhibitors on growth, cell signaling, and survival of Sarc cells. (A) cSarc cells have been exposed to inhibitors (i) of B-cell receptor/BTK-signaling pathway, CCND1-dependent CDK4/6 cell-cycle promoters, or the depicted growth factor receptors and analyzed for cell growth in the MTT conversion assay. (B) Inhibitory effect of FGFR inhibitor on activation of the MEK-ERK and PI3K-AKT signaling pathways using the depicted phospho-specific antibodies. Inhibitors of receptors for PDGF-β, TGF-β, and NGF served as negative controls. (C) Pathway-specific inhibitory effect of the MEK- and PI3K-specific inhibitors on activation of MEK-ERK and PI3K-AKT signaling pathways. (D) FGFR inhibitor-mediated induction of apoptotic cell death as determined by generation of cleaved caspase 3 and PARP. (E) FGFR inhibitor-induced cell death detected by DNA fragmentation (terminal deoxynucleotidyltransferase-mediated dUTP nick end labeling) assay.