Figure 3.
Expression and proliferative capacity of the p.Pro784Thr, p.Ala119Thr, and p.Trp547* CSF3R variants. (A) Immunoblot analysis of receptor expression in untransfected (parental) Ba/F3 cells and Ba/F3 cells transfected with WT, p.Pro784Thr, p.Ala119Thr, and p.Trp547* CSF3R. (B) Proliferative responses of cells in panel A in response to varying concentrations of G-CSF. Cell numbers were quantified using a luminescence-based assay at 48 hours after culture in the indicated concentrations of G-CSF. EC50 values calculated by dynamic curve fitting of the data for WT, p.Pro784Thr, and p.Ala119Thr were 0.25 ± 0.11, 0.05 ± 0.01, and 1.16 ± 0.08, respectively, with P values of .034 and .004 for p.Pro784Thr and p.Ala119Thr relative to WT, respectively (determined by 1-way analysis of variance). Data shown are representative of 4 independent experiments.

Expression and proliferative capacity of the p.Pro784Thr, p.Ala119Thr, and p.Trp547* CSF3R variants. (A) Immunoblot analysis of receptor expression in untransfected (parental) Ba/F3 cells and Ba/F3 cells transfected with WT, p.Pro784Thr, p.Ala119Thr, and p.Trp547* CSF3R. (B) Proliferative responses of cells in panel A in response to varying concentrations of G-CSF. Cell numbers were quantified using a luminescence-based assay at 48 hours after culture in the indicated concentrations of G-CSF. EC50 values calculated by dynamic curve fitting of the data for WT, p.Pro784Thr, and p.Ala119Thr were 0.25 ± 0.11, 0.05 ± 0.01, and 1.16 ± 0.08, respectively, with P values of .034 and .004 for p.Pro784Thr and p.Ala119Thr relative to WT, respectively (determined by 1-way analysis of variance). Data shown are representative of 4 independent experiments.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal