Abstract
GATA-1 and NF-kB are important in megakaryocytopoiesis and pathogenesis of idiopathic myelofibrosis (IMF). Previous studies have shown that GATA-1 levels were normal in mRNA but were lower in protein content in CD61+ cells of patients with IMF, and we reported previously that GATA-1 protein levels were not altered in CD61+ cells. The current study includes more patients and also measured NF-kB levels. ELISA assay (GATA-1 and NF-kB ELISA kit, Panomics Inc. CA) was used to determine and compare the protein content of nuclear extracts of blood hematopoietic progenitor cells (CD34+) and megakaryocytes (CD61+ cells) from patients with IMF, patients with other myeloproliferative disorders, and with controls. CD61+ cells were obtained from 10-day cultures of blood CD34+ cells in serum-free culture medium with thrombopoietin and stem cell growth factor. Our study confirmed that GATA-1 protein levels are not altered in megakaryocytes (cultured CD 61+ cells) in patients with IMF (Fig. 1) and NF-kB levels are increased (Table 1). We conclude that GATA-1 protein levels are not altered in patients with IMF compared with controls.
GATA-1 Protein levels in CD61+ cells Cultured from Patients with IMF & Others
Table 1. NF-kB Levels (ng/2μg protein)
| IMF | Controls | |
|---|---|---|
| CD34+ cells | 1.39 ± 0.27 | 1.10 ± 0.24 |
| CD61+ cells | 1.54 ± 0.52 | 0.45 ± 0.22 |
| IMF | Controls | |
|---|---|---|
| CD34+ cells | 1.39 ± 0.27 | 1.10 ± 0.24 |
| CD61+ cells | 1.54 ± 0.52 | 0.45 ± 0.22 |
Disclosures: No relevant conflicts of interest to declare.
